D-SIM

JIYUJIEGOUZHAOMINGDECAISESANWEICENGXIXIANWEICHENGXIANGXITONGSHIYOULINGYUNYUXIANGUANGXUEJINGMIJIXIEYANJIUSUOGONGTONGDAZAO，YIZHONGTONGSHIJUBEIGAODESHIJIANFENBIANLVHECAISESANWEICENGXICHENGXIANGNENGLIDEXIANWEIXITONG，QILIYONGGAOKONGJIANPINLVDEJIEGOUGUANGCHANGDUIYANGPINJINXINGZHAOMING，NENGBAKUANCHANGCHENGXIANGZHONGDEXIANGMIANXINXIHEFEIXIANGMIANXINXIYOUXIAODIFENLI，CONGERDEDAOCENGXITU，JINERTONGGUORUANJIANKEZHONGGOUCHUYANGPINDESANWEIJIEGOUTUXIANG；CHUCIZHIWAI，JIEGOUZHAOMINGXIANWEIJINGHAIKEYIJINXINGGAOFENBIANCHENGXIANG。TONGGUOJIEGOUGUANGDUIYANGPINGAOKONGJIANPINPUXINXIDEDIAOZHI，RANHOUJINGGUOKONGJIANPINYUDEJIEDIAOHECHULI，KEYIHUODEYANGPINDEGAOFENBIANLVTUXIANG，DADAOLIANGBEIYUCHUANTONGGUANGXUEXIANWEIJINGDEFENBIANLV。

CHANPINTEXING：

1. SHIYONGDMDCHANSHENGJIEGOUZHAOMINGGUANG，SUDUKUAI，GENGSHIYONGYUHUOTISHENGWUXIBAODESHISHISANWEICHENGXIANGYANJIUHEKUAISUDONGTAIGUOCHENGGUANCE；

2. CAIYONGFEIXIANGGANLEDGUANGYUANZHAOMING，XIAOCHUXIANGGANZAOSHENGDUICHENGXIANGZHILIANGDEYINGXIANG，GUANGSUNSHANGHEGUANGPIAOBAIXIAOYINGXIAO；

3. XITONGJIEGOUJINCOU，CAIYONGMOKUAIHUASHEJI，FANGBIANGAIZHUANGSHENGJI、TUOZHANGONGNENG；

4. SANZHONGGUANCHACHENGXIANGMOSHI：

（1）PUTONGYINGGUANGXIANWEICHENGXIANGMOSHI；

（2） SANWEICENGXIMOSHI：YANGPINYANGUANGZHOUFANGXIANGSAOMIAO，QUANFENBIANLVXIAKEDA20CENG/MIAODESUDU，LIANXUBUZHUOYANGPINSANWEICENGXITUXIANG，QIEPIANCENGJIANJU200nm~1µmKEDIAO；

（3） ERWEICHAOFENBIANMOSHI：LIANGBEIYUYANSHEJIXIANDEKONGJIANFENBIANLV（YUE100nm）；

5. XITONGSHIYONGGUANCHADEBIAOJIYINGGUANGRANLIAO/YINGGUANGDANBAI（GONGCANKAO）：

（1）JIFABOZHANG405nm：Alexa Fluor 430, DAPI, CFP, SYTO 41, CellTracker Violet BMQC；

（2） JIFABOZHANG470nm：Alexa Fluor 488, APF, Calcein Green, GFP, eGFP, MitoTracker Green FM；

（3）JIFABOZHANG565nm：Alexa Fluor 546, Alexa Fluor 555, MitoTracker Red, BOBO-3, Texas Red；

（4）JIFABOZHANG625nm：Alexa Fluor 633, Alexa Fluor 647, TO-PRO-3, MitoTracker Deep Red FM；